Journal: Bioactive Materials

Article Title: Commensal microbiota–coated biohybrid implants induce antibiofilm, osteogenic, and immunomodulatory responses in a human 3D immunocompetent model

doi: 10.1016/j.bioactmat.2026.06.006

Figure Lengend Snippet: Commensal microflora coatings support adhesion and viability of human cells. (A) CLSM images showing the morphology and distribution of gingival fibroblasts, oral keratinocytes, and macrophages after 48 h on glass, Ti, and CHM. Scale bar: 100 μm. (B) Metabolic activity of (a) fibroblasts, (b) keratinocytes, and (c) macrophages after 48 h on the indicated surfaces. Cells were stained with green fluorescent Calcein AM to label viable cells and with red fluorescent propidium iodide (PI) to label nonviable (dead) cells. All experiments were conducted using three biological replicates (N = 3) and three technical replicates per group (n = 3). For CLSM, the biocompatibility assay was imaged at 10× magnification with a voxel size of 1.52 μm (x) × 1.52 μm (y) × 3 μm (z).

Article Snippet: Ti- and CHM-integrated tissues, after coculture under sterile or biofilm-infected conditions, were longitudinally sectioned at the tissue–implant interface and stained with Calcein AM and propidium iodide (Invitrogen, Germany) for 30 min.

Techniques: Activity Assay, Staining

Journal: Life Science Alliance

Article Title: The noncoding RNA GRAS1 interacts with NKAP and protects against DNA damage and cell death

doi: 10.26508/lsa.202403105

Figure Lengend Snippet: Representative images of cell cycle analysis by propidium iodide staining using flow cytometry of A549 cells treated with control GapmeR compared with GRAS1 GapmeR1 or GRAS1 GapmeR2 (left). Quantification of the proportion of cells in each cell cycle phase was performed from three independent experiments (right). Error bars represent SD in all panels; (ns) P > 0.05; (*) P ≤ 0.05; (***) P < 0.001 by two-tailed t test.

Article Snippet: Then cells were resuspended in 100 μl of 1X Annexin-binding buffer (10 mM HEPES adjusted to pH 7.4, 140 mM NaCl, and 2.5 mM CaCl2) with 5 μl of FITC-conjugated Annexin V (A13199; Life Technologies) and 2 μl of 1 mg/ml propidium iodide (40017; Biotium).

Techniques: Cell Cycle Assay, Staining, Flow Cytometry, Control, Two Tailed Test